| 质粒名称: | pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 115211 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | SUMO bacterial expression vector |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6087 bp |
| 完整质粒大小: | 10053 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PsmCas13,Cas13a from Prevotella sp. MA2016 |
| 物种来源: | Prevotella sp. MA2016 |
| 插入大小: | 3966 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | SUMO |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI was a gift from Feng Zhang (Addgene plasmid # 115211 ; http://n2t.net/addgene:115211 ; RRID:Addgene_115211) |
| 参考文献部分: | Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15. 10.1126/science.aaq0179 PubMed 29449508 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
 His6-TwinStrep-SUMO-BsaI质粒图谱.png)
载体简介
载体序列
LOCUS pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI 10050 bp ds-DNA circular SYN 20-SEP-2018
DEFINITION Expresses PsmCas13b from a SUMO-tagged bacterial expression
construct.
ACCESSION .
VERSION .
KEYWORDS pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10050)
AUTHORS Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang
F
TITLE Multiplexed and portable nucleic acid detection platform with Cas13,
Cas12a, and Csm6.
JOURNAL Science. 2018 Apr 27;360(6387):439-444. doi:
10.1126/science.aaq0179. Epub 2018 Feb 15.
PUBMED 29449508
REFERENCE 2 (bases 1 to 10050)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 20, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10050
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 1..25
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 40..62
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 81..98
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 108..125
/codon_start=1
/product="thrombin recognition and cleavage site"
/label=thrombin site
/translation="LVPRGS"
CDS 138..161
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 198..221
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 222..512
/codon_start=1
/gene="S. cerevisiae SMT3 (truncated)"
/product="cleavable ubiquitin-like protein tag"
/label=SUMO
/translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG"
primer_bind complement(4532..4550)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 4546..4593
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter complement(4768..4796)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(4823..4841)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4909..5013
/gene="bla"
/label=AmpR promoter
CDS 5014..5874
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5232..5251)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6045..6633
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6534..6553
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6787..6804
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 6819..6958
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(6884..6903)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7044..7066
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(7060..7251)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(8563..9645)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 9607..9626
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(9646..9723)
/gene="lacI"
/label=lacI promoter
primer_bind 9929..9948
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 10032..1
/label=T7
/note="T7 promoter, forward primer"
promoter 10032..10050
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
61 gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg gtgccgcgcg
121 gcagccatat ggctagctgg agccatccgc agtttgaaaa aggtggtggt agcggtggtg
181 gttcaggtgg tagtgcatgg tcacaccctc agtttgagaa aatgtcggac tcagaagtca
241 atcaagaagc taagccagag gtcaagccag aagtcaagcc tgagactcac atcaatttaa
301 aggtgtccga tggatcttca gagatcttct tcaagatcaa aaagaccact cctttaagaa
361 ggctgatgga agcgttcgct aaaagacagg gtaaggaaat ggactcctta agattcttgt
421 acgacggtat tagaatccaa gctgatcaga cccctgaaga tttggacatg gaggataacg
481 atattattga ggctcacaga gaacagattg gtggatctat gtctaaggag tgcaagaagc
541 agcgccagga gaagaagcgg agactgcaga aggccaattt ctccatctct ctgaccggca
601 agcacgtgtt cggcgcctac tttaatatgg ccagaacaaa ctttgtgaaa accatcaact
661 acatcctgcc tatcgccggc gtgaggggca actatagcga gaatcagatc aacaagatgc
721 tgcacgcact gttcctgatc caggcaggac gcaatgagga gctgaccaca gagcagaagc
781 agtgggagaa gaagctgcgg ctgaacccag agcagcagac caagtttcag aagctgctgt
841 tcaagcactt tccagtgctg ggaccaatga tggcagacgt ggcagatcac aaggcctatc
901 tgaacaagaa gaagagcacc gtgcagacag aggacgagac attcgccatg ctgaagggcg
961 tgtccctggc cgactgcctg gatatcatct gtctgatggc cgataccctg acagagtgta
1021 gaaacttcta cacacacaag gacccttata acaagccaag ccagctggcc gatcagtacc
1081 tgcaccagga gatgatcgcc aagaagctgg acaaggtggt ggtggcctcc aggcgcatcc
1141 tgaaggatag ggagggcctg tctgtgaatg aggtggagtt cctgaccggc atcgatcacc
1201 tgcaccagga ggtgctgaag gacgagttcg gcaacgccaa ggtgaaggat ggcaaagtga
1261 tgaaaacctt cgtggagtac gacgacttct acttcaagat ctccggcaag cggctggtga
1321 atggctacac agtgaccaca aaggacgata agcccgtgaa tgtgaacacc atgctgcctg
1381 ccctgtctga cttcggcctg ctgtacttct gcgtgctgtt tctgagcaag ccctatgcca
1441 agctgtttat cgatgaggtg cgcctgttcg agtactctcc ttttgacgat aaggagaaca
1501 tgatcatgtc tgagatgctg agcatctatc ggatcagaac accccggctg cacaagatcg
1561 actcccacga ttctaaggcc accctggcca tggacatctt cggcgagctg cggagatgtc
1621 ctatggagct gtataacctg ctggacaaga acgccggcca gccattcttt cacgatgagg
1681 tgaagcaccc aaactctcac acccccgacg tgagcaagcg gctgagatac gacgatcgct
1741 ttcctacact ggccctgcgg tatatcgatg agacagagct gttcaagcgg atcagatttc
1801 agctgcagct gggcagcttc cgctacaagt tttatgataa ggagaattgc atcgacggcc
1861 gggtgagagt gaggcgcatc cagaaggaga tcaacggcta cggcagaatg caggaggtgg
1921 ccgacaagag gatggataag tggggcgacc tgatccagaa gagggaggag cgcagcgtga
1981 agctggagca cgaggagctg tatatcaatc tggaccagtt cctggaggac accgccgatt
2041 ccacacctta cgtgaccgat agacggcccg cctataatat ccacgccaac cggatcggcc
2101 tgtactggga ggactcccag aatcctaagc agtacaaggt gtttgatgag aacggcatgt
2161 atatccccga gctggtggtg accgaggaca agaaggcccc tatcaagatg ccagcaccta
2221 ggtgtgcact gagcgtgtac gacctgccag ccatgctgtt ctacgagtat ctgcgggagc
2281 agcaggataa cgagtttccc agcgccgagc aggtcatcat cgagtacgag gacgattatc
2341 gcaagttctt taaggccgtg gccgagggca agctgaagcc cttcaagcgg cccaaggagt
2401 tcagggactt tctgaagaag gagtacccaa agctgcggat ggccgatatc cccaagaagc
2461 tgcagctgtt tctgtgcagc cacggcctgt gctacaacaa taagcccgag acagtgtatg
2521 agcggctgga cagactgacc ctgcagcacc tggaggagag agagctgcac atccagaaca
2581 ggctggagca ctaccagaag gacagagata tgatcggcaa taaggacaac cagtatggca
2641 agaagagctt ctccgatgtg cgccacggcg ccctggccag atacctggca cagagcatga
2701 tggagtggca gcccacaaag ctgaaggata aggagaaggg ccacgacaag ctgaccggcc
2761 tgaattataa cgtgctgaca gcctacctgg caacctatgg ccacccacag gtgcctgagg
2821 agggcttcac ccctagaaca ctggagcagg tgctgatcaa tgcccacctg atcggcggct
2881 ccaatccaca cccctttatc aacaaggtgc tggccctggg caataggaac atcgaggagc
2941 tgtacctgca ctatctggag gaggagctga agcacatcag gtcccgcatc cagtctctga
3001 gctccaaccc tagcgacaag gccctgtccg ccctgccatt catccaccac gatcggatga
3061 gataccacga gcgcaccagc gaggagatga tggccctggc cgcacggtat accacaatcc
3121 agctgccaga cggcctgttt acaccctaca tcctggagat cctgcagaag cactataccg
3181 agaacagcga tctgcagaac gccctgtctc aggacgtgcc tgtgaagctg aatccaacct
3241 gcaacgccgc ctacctgatc acactgttct atcagaccgt gctgaaggac aatgcccagc
3301 ccttctacct gtccgataag acctatacaa gaaacaagga cggcgagaag gccgagtctt
3361 tcagctttaa gagggcctac gagctgttct ctgtgctgaa caataacaag aaggacacat
3421 tcccctttga gatgatccct ctgtttctga ccagcgatga gatccaggag agactgtccg
3481 ccaagctgct ggacggcgat ggcaatcctg tgccagaagt gggagagaag ggcaagccag
3541 ccacagacag ccagggcaac accatctgga agaggcgcat ctactccgag gtggacgatt
3601 atgccgagaa gctgaccgac cgcgatatga agatctcctt caagggcgag tgggagaagc
3661 tgcctcggtg gaagcaggat aagatcatca agcggcggga cgagacacgg cggcagatgc
3721 gggacgagct gctgcagagg atgccacgct acatccggga catcaaggat aatgagagga
3781 cactgcggag atataagacc caggatatgg tgctgttcct gctggccgag aagatgttta
3841 ccaatatcat ctccgagcag tctagcgagt tcaactggaa gcagatgagg ctgtctaagg
3901 tgtgcaacga ggccttcctg agacagaccc tgaccttccg ggtgcccgtg acagtgggcg
3961 agacaacaat ctacgtggag caggagaata tgtctctgaa gaactacggc gagttctatc
4021 gctttctgac agacgatcgg ctgatgagcc tgctgaataa catcgtggag acactgaagc
4081 ccaatgagaa cggcgacctg gtcatcagac acacagacct gatgagcgag ctggccgcct
4141 acgaccagta taggtctacc atcttcatgc tgatccagag catcgagaac ctgatcatca
4201 caaataacgc cgtgctggac gatccagacg ccgatggctt ctgggtgaga gaggatctgc
4261 ccaagaggaa taactttgcc tccctgctgg agctgatcaa tcagctgaat aacgtggagc
4321 tgaccgacga tgagcgcaag ctgctggtgg ccatccggaa cgccttcagc cacaattcct
4381 acaacatcga tttttccctg atcaaggacg tgaagcacct gcccgaggtg gccaagggca
4441 tcctgcagca cctgcagtct atgctgggcg tggagatcac caagtaaggc cgcactcgag
4501 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
4561 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat
4621 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
4681 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
4741 agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat
4801 gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
4861 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
4921 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
4981 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
5041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
5101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
5161 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
5221 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
5281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
5341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
5401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
5461 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
5521 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
5581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
5641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
5701 gctgataaat ctggagccgg tgagcgtggg tcacgcggta tcattgcagc actggggcca
5761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
5821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
5881 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
5941 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
6001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
6061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
6121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
6181 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
6241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
6301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
6361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
6421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
6481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
6541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
6601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
6661 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
6721 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
6781 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
6841 acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg
6901 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
6961 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
7021 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
7081 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
7141 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
7201 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc
7261 cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct
7321 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca
7381 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt
7441 cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg
7501 gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc
7561 gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
7621 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
7681 ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga
7741 gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt
7801 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
7861 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
7921 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
7981 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa
8041 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
8101 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
8161 ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg
8221 tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
8281 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
8341 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
8401 tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc
8461 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
8521 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
8581 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
8641 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
8701 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
8761 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt
8821 atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc
8881 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
8941 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
9001 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
9061 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
9121 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
9181 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
9241 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
9301 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
9361 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
9421 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
9481 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
9541 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
9601 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
9661 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
9721 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
9781 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
9841 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
9901 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
9961 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga
10021 tcccgcgaaa ttaatacgac tcactatagg
//
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